Lancashire have now gone four games without a victory in the Friends Provident t20 North Division after losing a pulsating Roses clash at Headingley.
Yorkshire won with five balls to spare in front of a 10,200 crowd to lift them off the bottom of the table.
In an exciting match of fluctuating fortunes, the pendulum finally swung Yorkshire’s way in the 18th over when Steve Patterson picked up three wickets in five balls as Lancashire made a desperate bid to get the 41 they still required for victory with four wickets in hand.
The first ball brought a single off a no-ball and was followed by four leg-byes before Gareth Cross was caught on the boundary edge by a jubilant Tino Best.
Mark Chilton was held on the mid-wicket boundary by Jacques Rudolph but captain Glen Chapple hooked the next ball for six.
But it was his last scoring shot because he then holed out to Richard Pyrah at long-off to give Patterson career-best figures of 4-30.
Lancashire entered the penultimate over on 128-9 and Sajid Mahmood lashed Best for six over mid-wicket but in the final over he struck Pyrah straight to Herschelle Gibbs at long-off.
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