Captain Michael Vaughan had to retire hurt with a twisted knee as England's tricky start to their tour of Pakistan continued on the second morning at the Bagh-e-Jinnah.

Vaughan came in at number three after the loss of opener Marcus Trescothick for just one second-innings run against Pakistan A in England's final fixture before the first Test, set to begin in Multan on Saturday.

He faced just one ball before he sunk to the ground in pain making his ground at the non-striker's end and - after several minutes' treatment on the pitch - he limped off to be replaced by Kevin Pietersen.

The immediate suggestion from the England camp was that the injury may be a recurrence of the problem which struck Vaughan down in the Lord's nets last year and ruled him out of the first Test against New Zealand when Trescothick took over as captain.

This time, the captain's leg was heavily iced and strapped as physio Kirk Russell sought to assess the extent of his discomfort.

Vaughan came to the crease when Trescothick was eliminated, pinned lbw on the front-foot defence by Mohammad Asif and on his way back before his team managed to rub off a marginal deficit after the hosts had been finished off for 138 in reply to 127.

Things were to go from bad to worse too for England when Pietersen went for a first-ball duck before Andrew Strauss and Paul Collingwood recovered to a lunchtime 58 without further loss.

Strauss escaped a sharp chance at gully on eight and Collingwood went from 15 to 21 off the same bowler with a hooked six ''caught' by Asif who ran over the boundary in doing so.